HIV (I + II) Antibody Test (Ob kab)

HIV (I + II) Antibody Test (Ob kab)

Hom: Daim Ntawv Tsis Txiav

Hom Tshuaj: Bio-mapper

Yam khoom: RF0171

Qauv: zis

Tus kab mob AIDS, tseem hu ua human immunodeficiency virus (HIV), yog ib yam kab mob uas tuaj yeem tua T4 lymphocytes, qhov tseem ceeb tshaj plaws ntawm tib neeg lub cev tiv thaiv kab mob.Cov tshuaj tiv thaiv kab mob HIV (HIV AB) muaj nyob rau hauv cov ntshav ntawm cov neeg uas muaj tus kab mob HIV, txawm tias lawv muaj cov tsos mob los yog tsis.Yog li, kev kuaj mob HIV AB yog qhov qhia tau tseem ceeb rau kev kuaj mob HIV.Txhawm rau txiav txim siab seb tus neeg puas muaj tus kab mob HIV, txoj hauv kev ib txwm kuaj yog mus rau cov tsev kho mob kom kuaj ntshav HIV antibody.Tus qauv HIV Ab test yog ib qho tshuaj tiv thaiv kab mob ntshav.Muaj ntau txoj hauv kev rau kev tshuaj ntsuam HIV Ab hauv tsev thiab txawv teb chaws, uas tuaj yeem muab faib ua enzyme-txuas immunosorbent assay, agglutination assay thiab immunochromatography raws li cov qauv kev kuaj pom sib txawv.Hauv kev ua haujlwm zoo, enzyme txuas immunosorbent assay, gelatin agglutination test thiab ntau yam kev kuaj mob sai yog feem ntau siv.

 


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Khoom cim npe

Cov lus piav qhia ntxaws

Western blot (WB), strip immunoassay (LIATEK HIV Ⅲ), radioimmunoprecipitation assay (RIPA) thiab immunofluorescence assay (IFA).Cov txheej txheem kev xeem siv feem ntau siv hauv Suav teb yog WB.

(1) Western blot (WB) yog ib txoj kev sim siv dav hauv kev kuaj mob ntawm ntau yam kab mob.Raws li deb li qhov kev kuaj mob etiological ntawm HIV muaj kev txhawj xeeb, nws yog thawj qhov kev sim ua pov thawj siv los lees paub HIV cov tshuaj tiv thaiv kab mob.Cov txiaj ntsig tshawb pom ntawm WB feem ntau yog siv los ua "tus qauv kub" txhawm rau txheeb xyuas qhov zoo thiab qhov tsis zoo ntawm lwm txoj kev sim.
Cov txheej txheem kuaj xyuas:
Muaj hom kab mob HIV-1/2 sib xyaw thiab ib hom kab mob HIV-1 lossis HIV-2 hom.Ua ntej, siv HIV-1/2 sib xyaw reagent los kuaj.Yog tias cov tshuaj tiv thaiv tsis zoo, qhia tias cov tshuaj tiv thaiv kab mob HIV tsis zoo;Yog tias nws zoo, nws yuav qhia tias nws yog HIV-1 antibody zoo;Yog tias tsis ua raws li cov txheej txheem zoo, nws raug txiav txim tias qhov kev sim tshuaj tiv thaiv kab mob HIV tsis paub meej.Yog tias muaj ib qho kev qhia tshwj xeeb ntawm HIV-2, koj yuav tsum siv HIV-2 immunoblotting reagent los ua qhov kev kuaj mob HIV 2 antibody dua, uas qhia tau hais tias tsis zoo, thiab tshaj tawm tias HIV 2 antibody tsis zoo;Yog tias nws yog qhov zoo, nws yuav qhia tias nws yog serologically zoo rau HIV-2 antibody, thiab xa cov qauv mus rau lub teb chaws siv lub chaw soj nstuam soj ntsuam nucleic acid,
Qhov rhiab heev ntawm WB feem ntau tsis qis dua qhov kev ntsuam xyuas ua ntej, tab sis nws qhov tshwj xeeb yog siab heev.Qhov no feem ntau yog raws li kev sib cais, kev xav thiab kev ua kom huv ntawm cov khoom sib txawv HIV antigen, uas tuaj yeem kuaj cov tshuaj tiv thaiv kab mob sib txawv ntawm cov tshuaj antigen, yog li WB txoj kev siv tau los txheeb xyuas qhov tseeb ntawm qhov kev ntsuam xyuas ua ntej.Nws tuaj yeem pom los ntawm WB cov txiaj ntsig kev lees paub tias txawm hais tias cov tshuaj reagents nrog cov khoom zoo raug xaiv rau qhov kev ntsuam xyuas ua ntej, xws li peb tiam ELISA, tseem yuav muaj qhov tsis zoo, thiab cov txiaj ntsig raug tsuas yog tau los ntawm kev kuaj pom tseeb.
(2) Immunofluorescence assay (IFA)
IFA txoj kev yog kev lag luam, yooj yim thiab ceev, thiab tau pom zoo los ntawm FDA rau kev kuaj mob ntawm WB cov qauv tsis meej.Txawm li cas los xij, yuav tsum tau siv cov fluorescent microscopes kim heev, yuav tsum tau kawm cov kws kho mob zoo, thiab kev soj ntsuam thiab kev txhais tau yooj yim cuam tshuam los ntawm cov ntsiab lus tseem ceeb.Cov txiaj ntsig yuav tsum tsis txhob khaws cia rau lub sijhawm ntev, thiab IFA yuav tsum tsis txhob nqa tawm thiab siv rau hauv chav kuaj dav dav.
Daim ntawv qhia txog cov tshuaj tiv thaiv kab mob HIV
Cov txiaj ntsig ntawm kev kuaj pom tus kab mob HIV yuav tsum tau tshaj tawm hauv Daim Ntawv Txuas Ntxiv 3.
(1) Ua raws li HIV 1 cov tshuaj tiv thaiv zoo rau kev txiav txim siab, tshaj tawm "HIV 1 antibody zoo (+)", thiab ua txoj haujlwm zoo ntawm kev sib tham tom qab kuaj, tsis pub lwm tus paub thiab qhia txog qhov xwm txheej kis raws li xav tau.Ua raws li kab mob HIV 2 antibody zoo txiav txim, tshaj tawm "HIV 2 antibody zoo (+)", thiab ua ib txoj hauj lwm zoo ntawm kev sib tham tom qab kuaj, tsis pub lwm tus paub thiab qhia txog qhov xwm txheej kis raws li qhov xav tau.
(2) Ua raws li cov txheej txheem kev txiav txim siab tsis zoo ntawm tus kab mob HIV, thiab tshaj tawm "HIV antibody negative (-)".Nyob rau hauv cov ntaub ntawv uas xav tias "lub sij hawm lub qhov rais" kab mob, ntxiv HIV nucleic acid kuaj yog pom zoo kom ua ib tug meej kev kuaj mob sai li sai tau.
(3) Ua raws li cov txheej txheem rau cov tshuaj tiv thaiv kab mob HIV tsis meej, tshaj tawm "HIV cov tshuaj tiv thaiv kab mob tsis paub tseeb (±)", thiab sau tseg hauv cov lus hais tias "tos rov kuaj dua tom qab 4 lub lis piam".

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