TOXO IgG/IgM Rapid Test

Kev tshuaj xyuas txoj kev
Muaj peb txoj kev kuaj mob tseem ceeb rau toxoplasmosis: kev kuaj kab mob, kev kuaj kab mob tiv thaiv kab mob thiab kev kuaj mob molecular.Kev kuaj kab mob feem ntau suav nrog kev kuaj mob histological, tsiaj inoculation thiab cais tawm, thiab kab lis kev cai ntawm tes.Cov txheej txheem serological diagnostic muaj xws li dye test, indirect hemagglutination test, indirect immunofluorescence antibody test thiab enzyme linked immunosorbent assay.Kev kuaj mob molecular suav nrog PCR thev naus laus zis thiab nucleic acid hybridization thev naus laus zis.
Kev kuaj cev xeeb tub ntawm cov niam uas muaj menyuam muaj xws li kev kuaj mob hu ua TORCH.TORCH yog kev sib xyaw ua ke ntawm thawj tsab ntawv ntawm lub npe Askiv ntawm ntau yam kab mob.Tsab ntawv T yog rau Toxoplasma gondii.(Lwm tsab ntawv sawv cev rau tus kab mob syphilis, tus kab mob rubella, cytomegalovirus thiab tus kab mob herpes simplex feem.)
Tshawb xyuas txoj cai
Kev kuaj kab mob
1. Direct microscopic soj ntsuam ntawm tus neeg mob cov ntshav, pob txha pob txha los yog cerebrospinal kua, pleural thiab ascites, hnoos qeev, bronchoalveolar lavage kua, aqueous lom zem, kua amniotic, thiab lwm yam. rau smears, los yog lymph nodes, nqaij, daim siab, placenta thiab lwm yam nyob rau hauv cov ntaub so ntswg los yog cov ntaub so ntswg jiscos. s, tab sis tus nqi zoo tsis siab.Nws kuj tseem tuaj yeem siv rau kev tiv thaiv kab mob ncaj qha txhawm rau txhawm rau kuaj Toxoplasma gondii hauv cov ntaub so ntswg.
2. Tsiaj inoculation los yog nqaij kab lis kev cai coj lub cev kua los yog cov ntaub so ntswg ncua mus kuaj thiab inoculate nws mus rau hauv lub plab kab noj hniav ntawm nas.Cov kab mob tuaj yeem tshwm sim thiab cov kab mob tuaj yeem pom.Thaum thawj tiam ntawm inoculation yog tsis zoo, nws yuav tsum tau dhau mus rau blindly rau peb zaug.Los yog rau kab lis kev cai ntawm cov ntaub so ntswg (lub raum liab lossis lub raum npua) kom cais tawm thiab txheeb xyuas Toxoplasma gondii.
3. DNA hybridization thev naus laus zis Cov kws tshawb fawb hauv tsev tau siv 32P daim ntawv lo sojntsuam uas muaj cov DNA tshwj xeeb ntawm Toxoplasma gondii thawj zaug los ua cov tshuaj molecular hybridization nrog cov hlwb lossis cov ntaub so ntswg DNA hauv cov neeg mob cov ntshav peripheral, thiab qhia tau hais tias cov hybridization bands tshwj xeeb los yog cov pob qij txha tau zoo.Ob qhov tshwj xeeb thiab rhiab heev tau siab.Tsis tas li ntawd, polymerase saw cov tshuaj tiv thaiv (PCR) kuj tau tsim nyob rau hauv Suav teb los kuaj tus kab mob, thiab muab piv nrog kev sojntsuam hybridization, tshuaj tiv thaiv tsiaj thiab tshuaj tiv thaiv kab mob, nws qhia tau hais tias nws yog qhov tshwj xeeb, rhiab thiab ceev.
Immunological kuaj
1. Antigens siv los kuaj cov tshuaj tiv thaiv kab mob feem ntau suav nrog tachyzoite soluble antigen (cytoplasmic antigen) thiab membrane antigen.Cov tshuaj tiv thaiv ntawm tus qub tau tshwm sim ua ntej (kuaj los ntawm kev kuaj staining thiab indirect immunofluorescence test), thaum lub sij hawm tom qab tshwm sim (kuaj los ntawm indirect hemagglutination test, thiab lwm yam).Nyob rau tib lub sijhawm, ntau txoj kev tshawb nrhiav tuaj yeem ua lub luag haujlwm ntxiv thiab txhim kho qhov kev kuaj pom.Vim tias Toxoplasma gondii tuaj yeem muaj nyob hauv tib neeg lub hlwb tau ntev, nws nyuaj rau kev paub qhov txawv ntawm tus kab mob tam sim no lossis yav dhau los los ntawm kev kuaj cov tshuaj tiv thaiv.Nws tuaj yeem raug txiav txim raws li cov tshuaj tiv thaiv kab mob titer thiab nws cov kev hloov pauv hloov pauv.
2. Kev kuaj xyuas antigen yog siv los txheeb xyuas cov kab mob (tachyzoites lossis cysts) hauv cov hlwb, cov tshuaj metabolites lossis cov khoom lysis (ntshav antigens) hauv cov ntshav thiab cov kua hauv lub cev los ntawm txoj kev tiv thaiv kab mob.Nws yog ib txoj hauv kev txhim khu kev qha rau kev kuaj mob ntxov thiab kev kuaj mob tseeb.Cov kws tshawb fawb hauv tsev thiab txawv teb chaws tau tsim McAb ELISA thiab qhaub cij ELISA ntawm McAb thiab multiantibody txhawm rau txheeb xyuas cov tshuaj tiv thaiv kab mob hauv cov ntshav ntawm cov neeg mob mob, nrog rau rhiab heev ntawm 0.4 μ G / ml ntawm antigen.