Cov lus piav qhia ntxaws
Xav txog tej yam ntaub ntawv ntawm tib neeg keeb kwm li kis tau thiab tswj lawv siv cov txheej txheem biosafety.
Plasma
1. Sau cov ntshav mus rau hauv lub raj mis, xiav lossis ntsuab saum toj sau cov raj (muaj EDTA, citrate lossis heparin, raws li hauv Vacutainer® ) los ntawm veinpuncture.
2. Sib cais cov ntshav los ntawm centrifugation.
3. Ua tib zoo tshem cov plasma mus rau hauv lub raj tshiab pre-labeled.
Serum
1. Sau cov ntshav mus rau hauv cov hlab ntshav liab (tsis muaj cov tshuaj tiv thaiv coagulants hauv Vacutainer®) los ntawm veinpuncture.
2. Cia cov ntshav txhaws.
3.Separate cov ntshav los ntawm centrifugation.
4. Ua tib zoo tshem cov ntshav mus rau hauv lub raj tshiab pre-labeled.
5.Kuaj kuaj kom sai li sai tau tom qab sau.Khaws cov khoom ntawm qhov kub ntawm 2 ° C mus rau 8 ° C yog tias tsis kuaj tam sim ntawd.
6. Khaws cov qauv ntawm 2 ° C txog 8 ° C mus txog 5 hnub.Cov qauv yuav tsum tau khov ntawm -20 ° C kom khaws cia ntev dua
Ntshav
Cov ntshav tag nrho tuaj yeem muab tau los ntawm ob tus ntiv tes ntiv tes puncture lossis veinpuncture.Tsis txhob siv cov ntshav hemolized los kuaj.Tag nrho cov ntshav kuaj yuav tsum muab cia rau hauv tub yees (2 ° C-8 ° C) yog tias tsis kuaj tam sim ntawd.Cov qauv yuav tsum raug kuaj hauv 24 teev ntawm kev sau. Tsis txhob muaj ntau lub voj voog khov-thaw.Ua ntej kev sim, nqa cov qauv khov rau hauv chav sov maj mam thiab sib tov maj mam.Cov qauv uas muaj qhov pom ntawm qhov teeb meem yuav tsum tau qhia meej los ntawm centrifugation ua ntej kuaj.
TSEEM CEEB
Kauj Ruam 1: Nqa cov qauv thiab kuaj cov khoom rau hauv chav tsev kub yog tias tso rau hauv tub yees lossis khov.Thaum thawed, sib tov cov qauv zoo ua ntej kuaj.
Kauj ruam 2: Thaum npaj txhij mus kuaj, qhib lub hnab ntawm lub thais thiab tshem tawm ntaus ntawv.Muab cov cuab yeej kuaj rau ntawm qhov chaw huv, tiaj tus.
Kauj ruam 3: Nco ntsoov sau lub cuab yeej nrog tus lej ID.
Kauj Ruam 4: Rau kev kuaj ntshav tag nrho - Siv 1 tee ntshav tag nrho (kwv yees li 30-35 µL) rau hauv cov qauv zoo.- Tom qab ntawd ntxiv 2 dropS (li 60-70 µL) ntawm Sample Diluent tam sim ntawd.