SUMMARY THIAB EXPLANATION THEM NYIAJ
Kev mob raws plab yog ib qho ntawm cov hauv paus ntsiab lus ua rau kev mob me nyuam yaus thiab kev tuag thoob ntiaj teb, ua rau 2.5 lab tus neeg tuag txhua xyoo.Tus kab mob rotavirus yog qhov ua rau mob raws plab heev hauv cov menyuam mos thiab cov menyuam yaus hnub nyoog qis dua 5 xyoos, suav txog 40% -60% ntawm mob plab hnyuv loj thiab ua rau kwv yees li 500,000 tus menyuam tuag txhua xyoo.Thaum muaj hnub nyoog tsib xyoos, yuav luag txhua tus menyuam hauv ntiaj teb tau kis tus kabmob rotavirus tsawg kawg ib zaug.Nrog rau cov kab mob tom ntej, qhov dav dav, heterotypic antibody teb yog elicited;yog li ntawd, cov neeg laus tsis tshua muaj kev cuam tshuam.
Txog hnub no xya pawg ntawm rotaviruses (pab pawg AG) tau raug cais tawm thiab
tus yam ntxwv.Pawg A rotavirus, feem ntau rotavirus, ua rau ntau tshaj 90% ntawm tag nrho cov kab mob Rotavirus hauv tib neeg.Rotavirus feem ntau kis tau los ntawm txoj kev fecaloral, ncaj qha los ntawm ib tus neeg mus rau lwm tus.Tus kab mob titers hauv cov quav mus txog qhov siab tshaj plaws sai tom qab pib mob, ces poob qis.Lub sij hawm incubation ntawm tus kab mob rotavirus feem ntau yog ib mus rau peb hnub thiab nws ua raws li gastroenteritis nrog lub sijhawm nruab nrab ntawm peb mus rau xya hnub.Cov tsos mob ntawm tus kab mob muaj xws li mob me, dej raws plab mus rau mob raws plab nrog kub taub hau thiab ntuav.
Kev kuaj mob ntawm tus kab mob rotavirus tuaj yeem ua tau tom qab kuaj mob gastroenteritis vim qhov ua rau mob plab hnyav hauv cov menyuam yaus.Tsis ntev los no, kev kuaj mob tshwj xeeb ntawm tus kab mob rotavirus tau dhau los ntawm kev kuaj pom tus kab mob antigen hauv cov quav los ntawm cov txheej txheem immunoassay xws li latex agglutination assay, EIA, thiab lateral flow chromatographic immunoassay.
Rotavirus Ag Rapid Test yog ib qho lateral flow chromatographic immunoassay uas siv ib khub ntawm cov tshuaj tiv thaiv tshwj xeeb txhawm rau txheeb xyuas cov kab mob rotavirus hauv qhov quav.Qhov kev sim tuaj yeem ua tiav yam tsis muaj cov cuab yeej kuaj sim, thiab cov txiaj ntsig muaj nyob hauv 15 feeb.
PRINCIPLE
Rotavirus Ag Rapid Test yog lateral flow chromatographic immunoassay.Cov kab ntawv xeem muaj xws li: 1) ib lub burgundy coloured conjugate ncoo uas muaj monoclonal anti-rotavirus antibody conjugated nrog colloidal kub (anti-rotavirus conjugates) thiab ib tug tswj antibody conjugated nrog colloidal kub, 2) ib tug nitrocellulose membrane strip uas muaj ib tug xeem kab (T kab) thiab kab tswj (C kab).T kab yog pre-coated nrog lwm monoclonal anti-rotavirus antibody, thiab C kab yog pre-coated nrog ib tug tswj kab antibody.
Thaum lub ntim txaus ntawm cov khoom xa tawm tau muab xa mus rau hauv cov qauv zoo ntawm cov ntawv xeem, cov qauv txav los ntawm kev ua haujlwm capillary thoob plaws lub cassette.Rotavirus Ag, yog muaj nyob rau hauv cov qauv, yuav khi rau cov anti-rotavirus conjugates.Tom qab ntawd cov immunocomplex raug ntes rau ntawm daim nyias nyias los ntawm cov tshuaj tiv thaiv kab mob ua ntej coated rotavirus uas tsim cov kab burgundy xim T, qhia tau tias muaj cov txiaj ntsig zoo ntawm cov kab mob rotavirus.Kev tsis muaj T kab qhia tias qhov concentration ntawm rotavirus Ag hauv cov hnoos qeev yog qis dua qib kuaj pom, qhia txog qhov tshwm sim tsis zoo ntawm rotavirus.Qhov kev sim no muaj kev tswj hwm sab hauv (C kab), uas yuav tsum pom cov kab xim burgundy ntawm cov tshuaj tiv thaiv kab mob ntawm cov tshuaj tiv thaiv kab mob, tsis hais txog kev loj hlob xim ntawm T kab.Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
