Cov lus piav qhia ntxaws
Kev kuaj pom ntawm yaj pox kab mob antibody yog tsim los ntawm microplate pre-coated nrog yaj pox nuclear protein antigen, enzyme markers thiab lwm yam kev txhawb nqa reagents, thiab lub hauv paus ntsiab lus ntawm enzyme-txuas immunoassay (ELISA) yog siv los kuaj cov yaj pox antibody hauv cov yaj cov ntshav.Thaum lub sij hawm sim, cov tshuaj tswj cov ntshav thiab cov qauv uas yuav tsum tau kuaj xyuas yog ntxiv rau lub phaj microplate, thiab yog tias cov qauv muaj cov yaj pox antibody tom qab incubation, nws yuav raug khi rau antigen ntawm lub phaj microplate, thiab lwm yam khoom uas tsis yog khi yuav raug tshem tawm tom qab ntxuav;Tom qab ntawd ntxiv cov enzyme marker rau tshwj xeeb khi rau antigen-antibody complex ntawm microplate phaj;Cov cim enzyme uas tsis muaj kev cuam tshuam tau raug tshem tawm los ntawm kev ntxuav, thiab TMB substrate tov tau ntxiv rau hauv qhov dej, thiab cov khoom xiav tau tsim los ntawm cov tshuaj tiv thaiv ntawm microplate conjugates, thiab xim qhov tob tau zoo sib raug zoo nrog cov tshuaj tiv thaiv tshwj xeeb uas muaj nyob hauv cov qauv.Tom qab qhov kev daws teeb meem raug ntxiv los txiav tawm cov tshuaj tiv thaiv, cov khoom tig daj;Tus nqi absorbance nyob rau hauv txhua qhov tshuaj tiv thaiv zoo yog txiav txim los ntawm tus nyeem ntawv microplate ntawm lub wavelength ntawm 450 nm los txiav txim seb tus qauv puas muaj cov tshuaj tiv thaiv yaj pox.