SUMMARY THIAB EXPLANATION THEM NYIAJ
Malaria yog kab mob yoov tshaj cum, hemolytic, febrile uas kis tau ntau tshaj 200 lab tus tib neeg thiab tua ntau dua 1 lab tus tib neeg hauv ib xyoos.Nws tshwm sim los ntawm plaub hom Plasmodium: P. falciparum, P. vivax, P. ovale, thiab P. malariae.Cov plasmodia no kis tau thiab rhuav tshem tib neeg erythrocytes, ua kom txias, ua npaws, anemia, thiab splenomegaly.P. falciparum ua rau muaj kab mob ntau dua li lwm hom plasmodial thiab suav rau feem ntau cov neeg mob malaria tuag.P. falciparum thiab P. vivax yog cov kab mob ntau tshaj plaws, txawm li cas los xij, muaj ntau qhov sib txawv ntawm thaj chaw hauv hom kev faib tawm.
Kev lig kev cai, malaria raug kuaj los ntawm kev ua qauv qhia ntawm cov kab mob ntawm Giemsa stained tuab smears ntawm peripheral cov ntshav, thiab ntau hom plasmodium yog qhov txawv ntawm lawv cov tsos ntawm cov kab mob erythrocytes.Cov txheej txheem muaj peev xwm ntawm kev kuaj mob kom raug thiab txhim khu kev qha, tab sis tsuas yog thaum ua los ntawm cov kws tshaj lij microscopists siv cov txheej txheem teev tseg, uas nthuav tawm cov teeb meem loj rau thaj chaw deb thiab tsis zoo hauv ntiaj teb.
Malaria Pf/Pv Ag Rapid Test yog tsim los daws cov teeb meem no.Nws siv cov tshuaj tiv thaiv kab mob tshwj xeeb rau P. falciparum Histidine Rich Protein-II (pHRP-II) thiab rau P. vivax Lactate Dehydrogenase (Pv-LDH) txhawm rau kuaj pom thiab sib txawv ntawm kev kis kab mob P. falciparum thiab P. vivax.Qhov kev ntsuam xyuas tuaj yeem ua tau los ntawm cov neeg ua haujlwm tsis muaj kev cob qhia lossis cov neeg ua haujlwm tsawg, tsis muaj cov cuab yeej kuaj
PRINCIPLE
Malaria Pf/Pv Ag Rapid Test yog lateral flow chromatographic immunoassay.Cov khoom siv ntawm cov strips kuaj muaj xws li: 1) ib lub burgundy xim conjugate ncoo uas muaj nas anti-Pv-LDH antibody conjugated nrog colloid kub (Pv-LDH-kub conjugates) thiab nas anti-pHRP-II antibody conjugated nrog colloid kub (pHRP-II-kub conjugates), 2) ib tug bandose strips (Pv-LDH-kub conjugates) band).Pv band yog pre-coated nrog lwm tus nas anti-Pv-LDH cov tshuaj tiv thaiv tshwj xeeb rau kev kuaj pom tus kab mob Pv, Pf band yog precoated nrog polyclonal anti-pHRP-II cov tshuaj tiv thaiv rau kev kuaj pom tus kab mob Pf, thiab C band yog coated nrog tshis anti-nas IgG.
Thaum lub sij hawm ntsuam xyuas, ib qho txaus ntim ntawm cov ntshav kuaj tau muab xa mus rau hauv cov qauv zoo (S) ntawm cov ntawv xeem, lub lysis tsis yog ntxiv rau qhov tsis zoo (B).Cov tsis muaj cov tshuaj ntxuav tes uas lyses cov qe ntshav liab thiab tso tawm ntau yam antigens, uas txav los ntawm capillary kev txiav txim siab hla cov sawb tuav hauv lub cassette.Pv-LDH yog muaj nyob rau hauv cov qauv yuav khi rau Pv-LDH-kub conjugates.Lub immunocomplex yog ces ntes tau ntawm daim nyias nyias los ntawm pre-coated anti-Pv-LDH antibody, tsim ib tug burgundy xim Pv band, qhia ib tug Pv zoo xeem.Xwb, pHRP-II yog muaj nyob rau hauv cov qauv yuav khi rau pHRP-II-kub conjugates.Lub immunocomplex yog ces ntes tau ntawm daim nyias nyias los ntawm pre-coated anti-pHRP-II cov tshuaj tiv thaiv, tsim ib tug burgundy xim Pf band, qhia tau hais tias ib tug Pf zoo xeem.Tsis muaj ib qho kev xeem qhab nia qhia tau qhov tsis zoo.Qhov kev ntsuam xyuas muaj kev tswj hwm sab hauv (C band) uas yuav tsum tau nthuav tawm cov xim burgundy ntawm cov tshuaj tiv thaiv ntawm tshis anti-nas IgG / nas IgG (anti-Pv-LDH thiab anti-pHRPII)-kub conjugates tsis hais txog kev loj hlob ntawm cov xim ntawm ib qho ntawm cov qhab nia xeem.Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
