SUMMARY THIAB EXPLANATION THEM NYIAJ
Visceral leishmaniasis, los yog Kala-azar, yog ib qho kev kis kab mob los ntawm ntau hom kab mob L. donovani.Tus kab mob no tau kwv yees los ntawm Lub Koom Haum Saib Xyuas Kev Noj Qab Haus Huv Ntiaj Teb (WHO) kom cuam tshuam txog kwv yees li 12 lab tus tib neeg hauv 88 lub tebchaws.Nws kis tau mus rau tib neeg los ntawm kev tua ntawm Phlebotomus sandflies, uas kis tau tus kab mob los ntawm kev noj cov tsiaj txhu.Txawm hais tias nws yog ib tus kab mob pom nyob rau hauv cov teb chaws txom nyem, nyob rau yav qab teb Europe, nws tau dhau los ua tus kab mob kis rau cov neeg mob AIDS.Kev txheeb xyuas cov kab mob L. donovani los ntawm cov ntshav, pob txha pob txha, daim siab, cov qog nqaij hlav los yog tus po yog qhov qhia meej txog kev kuaj mob.Serological nrhiav pom ntawm anti-L.donovani IgM pom tau tias yog ib qho cim zoo rau tus mob Visceral leishmaniasis.Cov kev ntsuam xyuas siv hauv tsev kho mob suav nrog ELISA, fluorescent antibody lossis direct agglutination tests 4-5.Tsis ntev los no, kev siv L. donovani cov protein tshwj xeeb hauv kev sim tau txhim kho qhov rhiab heev thiab tshwj xeeb heev.
Lub Leishmania IgG/IgM Combo Rapid Test yog ib qho kev ntsuam xyuas cov protein raws li serological, uas kuaj pom IgG thiab IgM cov tshuaj tiv thaiv rau L. Donovani ib txhij.Qhov kev ntsuam xyuas muab cov txiaj ntsig zoo hauv 15 feeb yam tsis muaj cov cuab yeej siv.
PRINCIPLE
Leishmania IgG / IgM Rapid Test yog lateral flow chromatographic immunoassay.Cov ntawv xeem muaj xws li: 1) ib lub burgundy xim conjugate ncoo uas muaj recombinant L. donovani antigen conjugated nrog colloid kub (Leishmania conjugates) thiab luav IgG-kub conjugates, 2) ib tug nitrocellulose membrane strips muaj ob lub xeem bands (T1 thiab T2 bands) thiab tswj band (C band).T1 band yog pre-coated nrog monoclonal anti-tib neeg IgM rau kev kuaj pom ntawm anti-L.donovani IgM, T2 band yog precoated nrog reagents rau kev tshawb pom ntawm anti-L.donovani IgG, thiab C band yog precoated nrog tshis anti luav IgG.
Thaum lub ntim ntim txaus ntawm cov qauv kuaj tau muab xa mus rau hauv cov qauv zoo ntawm lub cassette, cov qauv txav mus los ntawm kev ua haujlwm capillary hla lub cassette.L. donovani IgM yog muaj nyob rau hauv cov qauv yuav khi rau cov Leishmania conjugates.Lub immunocomplex yog ces ntes tau ntawm daim nyias nyias los ntawm pre-coated anti-tib neeg IgM antibody, tsim ib tug burgundy xim T1 band, qhia ib tug L. donovani IgM zoo xeem.L. donovani IgG yog muaj nyob rau hauv cov qauv yuav khi rau Leishmania conjugates.Lub immunocomplex yog ces ntes tau los ntawm pre-coated reagents ntawm daim nyias nyias, tsim ib tug burgundy xim T2 band, qhia tau hais tias ib tug L. donovani IgG zoo xeem.
Tsis muaj T bands (T1 thiab T2) qhia tau tias qhov tshwm sim tsis zoo.Qhov kev sim no muaj kev tswj hwm sab hauv (C band) uas yuav tsum tau nthuav tawm cov xim burgundy ntawm cov tshuaj tiv thaiv kab mob ntawm tshis anti luav IgG / luav IgG-kub conjugate tsis hais txog xim kev loj hlob ntawm ib qho ntawm T bands.Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
