HCV (CMIA)

Lub pathogenesis ntawm kab mob siab C tseem tsis meej.Thaum HCV rov ua dua hauv daim siab, nws ua rau muaj kev hloov pauv hauv cov qauv thiab kev ua haujlwm ntawm lub siab hlwb lossis cuam tshuam nrog kev sib txuas ntawm daim siab cell proteins, uas tuaj yeem ua rau degeneration thiab necrosis ntawm daim siab hlwb, qhia tias HCV ncaj qha ua rau lub siab puas tsuaj thiab ua lub luag haujlwm hauv pathogenesis.Txawm li cas los xij, ntau tus lej ntseeg tias cellular immunopathological cov tshuaj tiv thaiv tuaj yeem ua lub luag haujlwm tseem ceeb.Lawv pom tias kab mob siab C, zoo li kab mob siab B, feem ntau yog CD3 + infiltrating hlwb hauv nws cov ntaub so ntswg.Cytotoxic T cells (TC) tshwj xeeb tua cov hlwb ntawm HCV kab mob, uas tuaj yeem ua rau lub siab puas hlwb.

RIA los yog ELISA

Radioimmunodiagnosis (RIA) lossis enzyme-linked immunosorbent assay (ELISA) tau siv los kuaj xyuas cov tshuaj tiv thaiv HCV hauv cov ntshav.Xyoo 1989, Kuo et al.tsim ib txoj kev radioimmunoassay (RIA) rau anti-C-100.Tom qab ntawd, Ortho Company tau ua tiav qhov kev ntsuam xyuas enzyme-linked immunosorbent (ELISA) txhawm rau txhawm rau tiv thaiv C-100.Ob txoj hauv kev siv cov poov xab rov ua kom pom tus kab mob antigen (C-100-3, protein encoded los ntawm NS4, muaj 363 amino acids), tom qab ntxuav, nws yog coated nrog me me ntawm cov yas phaj qhov, thiab tom qab ntawd ntxiv nrog cov tshuaj ntsuam xyuas.Cov kab mob antigen tom qab ntawd ua ke nrog cov tshuaj tiv thaiv C-100 hauv cov ntshav kuaj.Thaum kawg, isotope lossis enzyme sau npe nas los tiv thaiv tib neeg lgG monoclonal antibody ntxiv, thiab cov substrate ntxiv rau kev txiav txim siab xim.