SUMMARY THIAB EXPLANATION THEM NYIAJ
Chikungunya yog ib qho kab mob tsis tshua muaj kis los ntawm kev kis tus kab mob Aedes aegypti yoov tshaj cum.Nws yog tus cwj pwm los ntawm pob khaus, ua npaws, thiab mob pob qij txha (arthralgias) uas feem ntau kav peb mus rau xya hnub.Lub npe yog muab los ntawm Makonde lo lus lub ntsiab lus "qhov twg khoov" nyob rau hauv kev siv rau lub stooped posture tsim los ntawm cov tsos mob ntawm tus mob caj dab.Nws tshwm sim thaum lub caij los nag
nyob rau hauv cov cheeb tsam sov ntawm lub ntiaj teb no, feem ntau nyob rau hauv teb chaws Africa, South-East Asia, yav qab teb Is Nrias teb thiab Pakistan.Cov tsos mob feem ntau yog qhov chaw kho mob tsis paub qhov txawv ntawm cov neeg pom hauv tus mob dengue.Tseeb tiag, ob tus kab mob dengue thiab chikungunya tau tshaj tawm hauv Is Nrias teb.Tsis zoo li dengue, hemorrhagic manifestations yog qhov tsawg thiab feem ntau tus kab mob yog ib qho kev txwv tus kheej tsis muaj mob febrile.Yog li ntawd nws tseem ceeb heev
txhawm rau txheeb xyuas qhov txawv ntawm tus kab mob dengue los ntawm tus kab mob CHIK.CHIK raug kuaj raws li kev soj ntsuam serological thiab kev cais tus kab mob hauv nas lossis kab lis kev cai.Ib qho IgM immunoassay yog txoj kev kuaj pom zoo tshaj plaws.Chikungunya IgG / IgM Rapid Test siv recombinant antigens muab los ntawm nws cov qauv protein, nws pom IgG / IgM anti-CHIK hauv cov neeg mob ntshav lossis ntshav hauv 20 feeb.Qhov kev xeem tuaj yeem ua los ntawm cov tsis tau kawm lossis
Cov neeg ua haujlwm tsawg kawg nkaus, tsis muaj cov cuab yeej kuaj sim cumbersome.
PRINCIPLE
Chikungunya IgG / IgM + NS1 antigen Rapid Test yog ib qho lateral flow chromatographic immunoassay.Cov ntawv xeem muaj IgG / IgM Sawb thiab NS1 strip.
IgG / IgM strip: 1) ib lub burgundy coloured conjugate ncoo uas muaj Chikungunya recombinant hnab ntawv antigens conjugated nrog colloid kub (dengue conjugates) thiab luav IgG-kub conjugates, 2) ib tug nitrocellulose membrane strip uas muaj ob lub xeem bands (G thiab M bands) thiab ib tug tswj band (C band).G band yog pre-coated nrog cov tshuaj tiv thaiv rau kev kuaj pom tus kab mob IgG anti-Chikungunya, M band yog coated nrog cov tshuaj tiv thaiv rau kev kuaj pom tus kab mob IgM anti-Chikungunya, thiab C band yog pre-coated nrog tshis tiv thaiv luav IgG.
NS1 strip: 1) burgundy coloured conjugate ncoo uas muaj nas anti-Chikungunya NS1 antigen conjugated nrog colloid kub (Chikungunya Ab conjugates), 2) ib tug nitrocellulose membrane strip uas muaj ib tug xeem band (T band) thiab ib tug tswj band (C band).T band yog pre-coated nrog luav anti-Chikungunya NS1 antigen, thiab C band yog pre-coated nrog tshis anti-nas IgG antibody.
IgG / IgM strip: Thaum lub ntim txaus ntawm cov qauv kuaj tau muab xa mus rau hauv cov qauv zoo ntawm cov ntawv xeem, cov qauv txav mus los ntawm kev ua haujlwm capillary thoob plaws lub cassette.IgG anti-Chikungunya tus kab mob yog tias muaj nyob rau hauv cov qauv yuav khi rau Chikungunya conjugates.Lub immunocomplex yog tam sim no ntes tau los ntawm cov reagent coated ntawm G band, tsim ib tug burgundy xim G band, qhia txog tus kab mob Chikungunya IgG qhov kev kuaj pom zoo thiab qhia txog kev kis tus kab mob tsis ntev los no lossis rov ua dua.IgM anti-Chikungunya tus kab mob, yog tias muaj nyob rau hauv cov qauv, yuav khi rau Chikungunya conjugates.Lub immunocomplex yog tom qab ntawd raug ntes los ntawm cov reagent pre-coated ntawm M band, tsim cov xim burgundy M band, qhia txog tus kab mob Chikungunya IgM qhov kev kuaj pom zoo thiab qhia txog tus kab mob tshiab.Tsis muaj ib qho kev xeem bands (G thiab M) qhia tias qhov tshwm sim tsis zoo.Qhov kev ntsuam xyuas muaj kev tswj hwm sab hauv (C band) uas yuav tsum muaj cov xim burgundy ntawm cov tshuaj tiv thaiv kab mob ntawm tshis tiv thaiv luav IgG /
luav IgG-kub conjugate tsis hais txog kev loj hlob xim ntawm ib qho ntawm T bands.Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
NS1 Sawb: Thaum lub ntim ntim txaus ntawm cov qauv kuaj tau muab tso rau hauv cov qauv zoo ntawm lub cassette, cov qauv txav mus los ntawm kev txiav txim siab capillary hla cov ntawv xeem.Chikungunya NS1 Ag yog muaj nyob rau hauv cov qauv yuav khi rau Chikungunya Ab conjugates.Lub immunocomplex yog ces ntes tau ntawm daim nyias nyias los ntawm precoated luav anti-NS1 antibody, tsim ib tug burgundy xim T band, qhia ib tug Chikungunya Ag zoo xeem.Kev tsis muaj T band qhia tias qhov tshwm sim tsis zoo.Qhov kev sim no muaj kev tswj hwm sab hauv (C band) uas yuav tsum tau nthuav tawm cov xim burgundy ntawm cov tshuaj tiv thaiv ntawm tshis los tiv thaiv nas IgG / nas IgG-kub conjugate tsis hais txog qhov muaj xim T band.Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
